This is a worksheet that helps students understand the process of PCR by actually writing out the details based on a provided template. This activity was created in response to the struggles that Introductory Biology 1113 students often have with question regarding why it takes 3 cycles of PCR to amplify the desired sequence with nothing extra. The questions could also be used as a prelab if distributed before lab. The PDF may be projected onto the whiteboard and students may fill in some of the answers as part of a classroom discussion. Unless the instructors choose to make this for points, you don't need to collect it unless students want feedback. It's more of a take-home resource to help students master the topic on their own. It's also helpful to have during office hours for those students who desire more help or deeper understanding. Students who can explain this worksheet generally don't have much trouble answering the lab questions or correctly answering quiz questions about PCR. Biology Learning Outcomes: 1.The Chemistry of Life a. Identify examples and list characteristics and general functions of the major classes of biological macromolecules (nucleic acids in particular) 3.Genetics h. Describe the experimental basis and select applications of recombinant DNA technology.